Tushratta (Sanskrit Tvesa-ratha, "his chariot charges") was a king of Mitanni at the end of the reign of Amenhotep III and throughout the reign of Akhenaten—approximately the late 14th century BC. He was the son of Shuttarna II. His sister Gilukhipa and his daughter Tadukhipa were married to the Egyptian pharaoh Amenhotep III; Tadukhipa later married Akhenaten who took over his father's royal harem.
He had been placed on the throne after the murder of his brother Artashumara. He was probably quite young at the time and was destined to serve as a figurehead only. But he managed to dispose of the murderer.
At the beginning of his reign, the Hittite King Suppiluliuma I, reconquered Kizzuwatna, then invaded the western part of the Euphrates valley and conquered the Amurru and Nuhašše in Hanigalbat. According to the Suppiluliuma-Shattiwaza treaty, Suppiluliuma had made a treaty with Artatama, a rival of Tushratta. Nothing is known of Artatama's previous life or connection, if any, to the royal family. The document calls him king of the Hurrians, while Tushratta is given the title of "King of Mitanni", which must have disagreed with Tushratta. Suppiluliuma started to plunder the lands of the west bank of the Euphrates river and he annexed Mount Lebanon. Tushratta threatened to raid beyond the Euphrates if even a single lamb or kid was stolen.
Suppiluliuma then recounts how the land of Isuwa on the upper Euphrates had seceded in the time of his grandfather. Attempts to conquer it failed. In the time of his father, other cities rebelled. Suppiluliumas claims to have defeated them, but the survivors fled to the territory of Isuwa that must have been part of Tushratta's realm. A clause to return fugitives was part of many treaties made at the time, so possibly the harbouring of fugitives by Isuwa formed the pretext for the Hittite invasion. A Hittite army crossed the border, entered Isuwa and returned the fugitives (or deserters or exile governments) to Hittite rule. "I freed the lands which I captured; they dwelt in their places. All the people whom I released rejoined their peoples and Hatti incorporated their territories," Suppiluliuma later boasted.