QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution and a highly accurate technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point. This standardized variant of native gel electrophoresis is used by biologists to isolate active or native metalloproteins in biological samples and to resolve properly and improperly folded metal cofactor-containing proteins or protein isoforms in complex protein mixtures. The high reproducibility and the high-yield electroelution of proteins performed by this technique strongly correlates with the polymerization time of the acrylamide (AA) gels.

As omics platform for quantitative biomedical approaches QPNC-PAGE may contribute to the development of metal-based drugs. Another important link addresses the investigation of the role of environmental contaminants like copper in the etiology of Alzheimer's disease (AD) because inorganic copper that cannot be detoxified completely by the liver, may be a major triggering agent in AD.