Princess Isabel Alfonsa María Teresa Antonia Cristina Mercedes Carolina Adelaida Rafaela of Bourbon-Two Sicilies, Infanta of Spain,Spanish: Isabel Alfonsa Maria Teresa Antonia Cristina Mercedes Carolina Adelaide Raphaela, Princesa de Borbón-Dos Sicilias, Infanta de España (10 October 1904 in Madrid, Kingdom of Spain – 18 July 1985 in Madrid, Kingdom of Spain) was a member of the House of Bourbon-Two Sicilies and a Princess of Bourbon-Two Sicilies by birth. Through her marriage to Count Jan Kanty Zamoyski, Isabel Alfonsa was a member of the Zamoyski noble family and a Countess Zamoyska.

Isabel Alfonsa was the third child of Prince Carlos of Bourbon-Two Sicilies and his first wife Mercedes, Princess of Asturias. Her mother was the Heiress Presumptive of the Spanish Throne from 11 September 1880 to 17 October 1904. Isabel Alfonsa's maternal grandparents were Alfonso XII of Spain and Maria Christina of Austria. Through her father, Isabel Alfonsa was a great-granddaughter of Ferdinand II of the Two Sicilies.

Isabel Alfonsa married Count Jan Kanty Zamoyski, seventh child and third son of Count Andrzej Zamoyski and his wife Princess Maria Carolina of Bourbon-Two Sicilies, on 9 March 1929 in Madrid. Isabel Alfonsa and Jan had four children:

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