Lucius Sempronius Atratinus (died 7 AD) was a Roman politician who was elected suffect consul in 34 BC. He is mentioned in Pro Caelio, a famous speech in defense of Marcus Caelius Rufus by Marcus Tullius Cicero.
Probably born a member of the patrician branch of the ancient Sempronia family, Atratinus was possibly adopted by Lucius Calpurnius Bestia, but did not assume his adopted father’s nomen gentile. In 56 BC he launched a prosecution against Marcus Caelius Rufus who had previously unsuccessfully attempted to prosecute Atratinus’s adopted father on bribery charges. Caelius had fallen out with his lover, Clodia, and she accused him of attempted poisoning. Other charges included the murder of an ambassador. She asked Atratinus to prosecute Caelius, which he was only too happy to do. Caelius was successfully defended by Marcus Tullius Cicero, and in his published Pro Caelio, Cicero claimed that Atratinus was being manipulated by Clodia to get revenge on Caelius for an affair gone wrong.
In 40 BC, Atratinus was elected praetor suffectus, as all the previously elected praetors had retired from office after the Treaty of Brundisium between Octavianus, Mark Antony and Lepidus. Late in 40 BC, he and his colleague Marcus Valerius Messalla Corvinus convened the Senate to introduce Herod the Great, who received the title of King of Judea. This same year he was elected to the role of Augur, one of the priests of ancient Rome, a position he held until his death in 7 AD.