Usui District (碓氷郡 Usui-gun?) is a former rural district located in Gunma Prefecture, Japan. All of the city of Annaka and a portion of the city of Takasaki were formerly part of the district.
Per a census conducted at the end of the Edo period, the area of Usui district contained 33 villages under the control of Annaka Domain, nine villages under Takasaki Domain, four villages under Yoshii Domain and 29 villages administered as tenryō directly by the Tokugawa shogunate. Usui District was established on December 7, 1878, and with the establishment of the municipality system on April 1, 1889 the area was organized into five towns (Annaka, Haraichi, Sakamoto, Matsuida and Itahana) and 13 villages