Takasaki Domain (高崎藩 Takasaki -han?) was a feudal domain under the Tokugawa shogunate of Edo period Japan, located in Kōzuke Province (modern-day Gunma Prefecture), Japan. It was centered on Takasaki Castle in what is now part of the city of Takasaki, Gunma. Takasaki was ruled through most of its history by a junior branch of the Matsudaira clan.
During the late Heian period, the area around what is now Takasaki was controlled by the Wada clan. During the Muromachi period, the Wada came under the service of the Uesugi clan, who held the post of Kantō kanrei; however in 1561, Wada Narishige, incensed over the appointment of Uesugi Kenshin to the post, defected to the Takeda. His son, Wada Nobunari, in turn came into the service of the Odawara Hōjō. During the Battle of Odawara in 1590, Toyotomi Hideyoshi dispatched an army led by Uesugi Kagekatsu and Maeda Toshiie and destroyed Wada Castle.
After Tokugawa Ieyasu took control over the Kantō region in 1590, he assigned Ii Naomasa, one of his most trusted Four Generals to nearby Minowa Castle, with revenues of 120,000 koku. However, in 1597, Ieyasu ordered Ii Naomasa to construct a new castle on the site of the ruins of Wada Castle, as the location controlled a strategic junction connecting the Nakasendō with the Mikuni Kaidō highways. Ii Naomasu relocated to the site in 1598, renaming it Takasaki, and bringing with him the population of Minowa to form the nucleus of a new castle town. This marked the beginning of Takasaki Domain.