Isaac Van Horne (January 13, 1754, Solebury Township, Pennsylvania – February 2, 1834, Zanesville, Ohio) was a member of the U.S. House of Representatives from Pennsylvania.
He was the eldest son of Bernard Van Horne and his first wife Sarah Van Pelt. He is descended of several Dutch families who came to America when New York was a Colony of the Netherlands. His father's family, sometimes spelled "Vanhoorn", have been identified with the European Noble Family of that name.
His paternal immigrant ancestor Christian Barentsen van Hoorn had a house lot at Wall Street and Broadway which is now part of Trinity Churchyard, and was Fire Marshal of Manhattan in the 1650s before moving to the area of Wilmington, Delaware. His grandmother, Alice Sleght Van Horn, may have been descended from Catherine Trico Rapalgie, one of whose daughters was reputed to be the first white child born in New Netherland. He was also reputed to be the 3x-great grand nephew of Peter Stuyvesant (c.1612—1672).
He apprenticed as a carpenter and cabinetmaker and served was justice of the peace for Solebury Township in Bucks County of Pennsylvania for several years as well as coroner of Bucks County four years.
In 1775, he was elected ensign of a company of militia, and appointed ensign in the Continental Army by the committee of safety in January 1776, assigned to Capt. John Beatty's Company in Col. Magaw's Regiment.