Haladaptatus paucihalophilus
| Haladaptatus paucihalophilus | |
|---|---|
| Scientific classification | |
| Domain: | Archaea |
| Phylum: | Euryarchaeota |
| Class: | Halobacteria |
| Order: | Halobacteriales |
| Family: | Halobacteriaceae |
| Genus: | Haladaptatus |
| Species: | H. paucihalophilus |
| Binomial name | |
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Haladaptatus paucihalophilus Savage et al 2007, emend. |
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Haladaptatus paucihalophilus is a halophilic archaeal species, originally isolated from a spring in Oklahoma. It uses a new pathway to synthesize glycine, and contains unique physiological features for osmoadaptation.
H. paucihalophilus was originally found in 2004, but was not classified as a species at the time; only the Halobacteriales were studied.H. paucihalophilus was isolated from the Zodletone Spring in Oklahoma. It was originally considered to have two different strains: DX253 and GY252. However, the two strains were later deemed a single species, since they have a 97.7% species similarity in 16S ribosomal RNA sequence analysis. To isolate H. paucihalophilus specifically, soil samples from the spring were taken and later inoculated onto a halophile-selective medium and then analyzed further after colony growth. Testing was done for Gram stain, carbon source, acid production, growth at minimal salt concentration, and antibiotic sensitivity. Also, PCR was performed with the primers A1F and UA1406R.H. paucihalophilus was named for its ability to grow in low-salt environments (pauci meaning small, halo meaning salt, philus meaning loving).
Most species within the Halobacteriaceae can be found in environments such as springs and marshes, that contain a high salt concentration. However, many of these archaeal species that have a high tolerance to salt may also exist in low-salt environments.H. paucihalophilus is capable of surviving and growing within a broad range of salt concentrations, so can also be found living in low-salt environments, much like Zodletone Spring.
On the basis of 16S ribosomal RNA sequencing H. paucihalophilus is similar to the species Halalkalicoccus tibetensis by 89.5-90.8% with the differences concentrated at the base pairs of 1-200 and 400-800. Differences with the phospholipid content in H. paucihalophilus when compared to other halophilic genera mainly constitutes the differentiation.
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