Elizabeth Boleyn was a lady-in-waiting at the court of Henry VIII of England. Through her marriage to Sir James Boleyn, she was the aunt of Henry VIII's second wife, Anne Boleyn. The two were not close, and Elizabeth Boleyn acted as her niece's gaoler when Queen Anne was arrested on charges of adultery, incest and conspiracy to kill the King.
Elizabeth Boleyn was one of the many relatives who benefitted from the success of her brother-in-law, Sir Thomas Boleyn, who was a successful ambassador and rose to the title Viscount Rochford in 1525. After Henry fell in love with Thomas' daughter, Anne, Thomas Boleyn was given the earldoms of Wiltshire and Ormonde, titles that his grandfather had held.
Despite this, there seems to have been long-running animosity between Elizabeth Boleyn and her niece. In 1536 five women were appointed to serve Queen Anne while she was imprisoned in the Tower and to report to Sir William Kingston, the Lieutenant of the Tower, and through him to the King's chief minister, Thomas Cromwell, all that the Queen said. These women included Elizabeth Boleyn; Queen Anne's aunt, Anne Shelton; Mary Kingston, the wife of Sir William Kingston, the Lieutenant of the Tower; Margaret Coffin, the wife of Queen Anne's Master of the Horse; and Elizabeth Stoner, wife of the King's Serjeant-at-Arms. Sir William Kingston described the five as "honest and good women", but Queen Anne said that it was "a great unkindness in the King to set such about me as I have never loved".