Bartolomé de la Cueva y Toledo (1499–1562) was a Spanish Roman Catholic Roman Catholic cardinal and bishop.
Bartolomé de la Cueva y Toledo was born in Cuéllar on August 24, 1499, the son of Francisco Fernández de la Cueva, 2nd Duke of Alburquerque and his wife Francisca de Toledo.
As a young man, before he took Holy Orders, he had an illegitimate son, Bartolomé de la Cueva. Following his ordination, he was a cleric in the Diocese of Segovia, and a canon of the cathedral chapter of Toledo Cathedral. He also worked for the Apostolic See in Spain. In 1525, he and his brother Luis accompanied Charles V, Holy Roman Emperor during the emperor's visit to Italy.
Cardinal de la Cueva did not participate in the Council of Trent. He was a friend of Ignatius of Loyola, joining Loyola's Sociedad de la Gracia at Santa Marta al Collegio Romano in Rome in 1543. He was also the patron of the first Jesuit church build in Rome by Michelangelo in 1544.
Pope Paul III made him a cardinal priest in the consistory of December 19, 1544. He received the red hat and the titular church of San Matteo in Via Merulana on May 5, 1546. As a cardinal, he often resided at the papal court in Rome. Charles V was annoyed that Pedro Pacheco, Bishop of Pamplona was not elevated to the cardinalate and he forbade the three Spaniards made cardinals on December 19, 1544 from wearing the cardinalate habit until Bishop Pacheco was also made a cardinal the next year.