Anne Hamilton, Countess of Huntly

Anne Hamilton, Countess of Huntly (c.1535 – after 17 April 1574), was a Scottish noblewoman and a member of the powerful Hamilton family which had a strong claim to the Scottish crown. Her father James Hamilton, Duke of Châtellerault, 2nd Earl of Arran was heir presumptive to the throne of Scotland after Mary, Queen of Scots prior to the birth of the latter's son Prince James in 1566. Anne was the wife of George Gordon, 5th Earl of Huntly, Lord Chancellor of Scotland and a chief conspirator during the reign of Queen Mary.

In her teens, Anne entered Marie of Guise's household as a lady-in-waiting and maid-of-honour.

Lady Anne was born in Scotland in about 1535, the eldest daughter of James Hamilton, Duke of Châtellerault, 2nd Earl of Arran and Lady Margaret Douglas. Her paternal grandparents were James Hamilton, 1st Earl of Arran and Lady Janet Beaton, and her maternal grandparents were James Douglas, 3rd Earl of Morton and Catherine Stewart, illegitimate daughter of King James IV of Scotland by his mistress Marion Boyd. The Hamiltons were, next to the legitimate royal Stewarts, the noblest family in Scotland, and therefore the strongest claimants to the throne due to Anne's great-grandfather James Hamilton, 1st Lord Hamilton having been the husband of Princess Mary Stewart, the sister of King James III. From the time of the death of John Stewart, Duke of Albany in 1536 until the birth of James Stewart, Duke of Rothesay in 1450; from April 1541 when the infant Rothesay died along with his new-born brother, Arthur until the birth of Queen Mary on 8 December 1542; and then following the death of King James V on 14 December 1542 until the birth of Queen Mary's son, Prince James in 1566, Anne's father was the heir presumptive to the Kingdom of Scotland. When Mary abdicated in 1567 in favour of her son James, he once again became next in line to the throne until his own death on 22 January 1575.