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Agrobacterium tumefaciens

Agrobacterium tumefaciens
Agrobacterium-tumefaciens.png
A. tumefaciens attaching itself to a carrot cell
Scientific classification
Kingdom: Bacteria
Phylum: Proteobacteria
Class: Alphaproteobacteria
Order: Rhizobiales
Family: Rhizobiaceae
Genus: Agrobacterium
Species: A. tumefaciens
Binomial name
Agrobacterium tumefaciens
Smith & Townsend, 1907
Synonyms
  • Bacterium tumefaciens Smith and Townsend 1907
  • Pseudomonas tumefaciens (Smith and Townsend 1907) Duggar 1909
  • Phytomonas tumefaciens (Smith and Townsend 1907) Bergey et al. 1923
  • Polymonas tumefaciens (Smith and Townsend 1907) Lieske 1928

Agrobacterium tumefaciens (updated scientific name Rhizobium radiobacter, synonym Agrobacterium radiobacter) is the causal agent of crown gall disease (the formation of tumours) in over 140 species of eudicots. It is a rod-shaped, Gram-negative soil bacterium. Symptoms are caused by the insertion of a small segment of DNA (known as the T-DNA, for 'transfer DNA', not to be confused with tRNA that transfers amino acids during protein synthesis, confusingly also called transfer RNA), from a plasmid, into the plant cell, which is incorporated at a semi-random location into the plant genome.

A. tumefaciens is an alphaproteobacterium of the family Rhizobiaceae, which includes the nitrogen-fixing legume symbionts. Unlike the nitrogen-fixing symbionts, tumor-producing Agrobacterium species are pathogenic and do not benefit the plant. The wide variety of plants affected by Agrobacterium makes it of great concern to the agriculture industry.

Economically, A. tumefaciens is a serious pathogen of walnuts, grape vines, stone fruits, nut trees, sugar beets, horse radish, and rhubarb.

To be virulent, the bacterium must contain a tumour-inducing plasmid (Ti plasmid or pTi), of 200 kb, which contains the T-DNA and all the genes necessary to transfer it to the plant cell. Many strains of A. tumefaciens do not contain a pTi.


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