2016 Challenger Ciudad de Guayaquil – Singles
| Singles | |
|---|---|
| 2016 Challenger Ciudad de Guayaquil | |
| Champion |
 Nicolás Kicker
|
| Runner-up |
 Arthur De Greef
|
| Final score | 6–3, 6–2 |
Gastão Elias was the defending champion but lost in the first round to Guilherme Clezar.
Nicolás Kicker won the title after defeating Arthur De Greef 6–3, 6–2 in the final.
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