The 2000–01 New York Knicks season was the 54th season of NBA basketball in New York City, New York. During the offseason, the Knicks acquired All-Star forward Glen Rice from the Los Angeles Lakers and Luc Longley from the Phoenix Suns. In their first season without Patrick Ewing, the Knicks remained a perennial playoff contender. At midseason, Mark Jackson returned to the Knicks as he was acquired from the Toronto Raptors in exchange for Chris Childs. The Knicks finished third in the Atlantic Division with a 48–34 record, earning the fourth seed in the Eastern Conference. Allan Houston and Latrell Sprewell were both selected for the 2001 NBA All-Star Game. In the playoffs, the Knicks failed to advance to the semifinals for the first time in ten years as they were beaten by the 5th-seeded Raptors in five games.

It would be Rice's only season in New York as he was traded to the Houston Rockets following the season. Also following the season, due to lingering injuries, Longley and Larry Johnson both retired.

After 15 years with the New York Knicks, due to chronological age and injuries, Patrick Ewing parted ways with the organization on September 20, 2000 in a 4-team trade which involves the Seattle SuperSonics, the Phoenix Suns, and the Los Angeles Lakers. In the deal, the Knicks acquire Travis Knight, Glen Rice, and a 2001 1st round draft pick (#27, traded to Vancouver) from the Lakers, Luc Longley from the Phoenix Suns, Lazaro Borrell, Vernon Maxwell, Vladimir Stepania, two 2001 2nd round picks (#s 39 and 43; neither player selected ever played in the NBA), and a 2002 1st round pick (#20, traded to Toronto) from Seattle. During regular season, the Knicks later traded Erick Strickland, a 2001 1st and 2nd round draft picks to the Vancouver Grizzlies for Othella Harrington. And then Chris Childs and a 2002 1st round pick to the Toronto Raptors for Muggsy Bogues and Mark Jackson.

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