2000–01 National Division One

2000–01 National Division One
Countries	England
Champions	Leeds Tykes
Runners-up	Worcester
Relegated	Orrell and Waterloo
Attendance	158,824 (average 908 per match)
Highest attendance	5,509 Leeds Tykes at home to Worcester on 1 April 2001
Lowest attendance	200 (x3) Birmingham & Solihull at home to Waterloo on 14 October 2000, Moseley on 16 December 2001 & Otley on 4 February 2001
Top point scorer	Sateki Tuipulotu (Worcester) 396 Points
Top try scorer	Graham Mackay (Leeds Tykes) 19 tries
← 1999–00 2001–02 →

The 2001–02 National Division One (previously known as the Allied Dunbar Premiership Two and renamed as the Jewson National Division One for sponsorship reasons) was the fourteenth full season of rugby union within the second tier of the English league system, currently known as the RFU Championship. New teams to the division included Bedford Blues who had been relegated from the Allied Dunbar Premiership 1999-00 while Otley and Birmingham & Solihull had been promoted from National League Two. This season also saw the introduction of the bonus points scoring system. The season would also herald a new change in venue for Moseley who moved from their traditional home at The Reddings to share the University of Birmingham rugby pitch at Bournbrook, with The Reddings being sold to property developers in order to generate funds to keep the club in existence.

Leeds Tykes, the champions, were promoted to the Allied Dunbar Premiership for season 2001–02. There was only one promotion place available and the runners–up Worcester remained in National League One while Orrell and Waterloo were relegated to the 2001–02 National Division Two.