1965 Wimbledon Championships – Women's Singles
| Women's Singles | |
|---|---|
| 1965 Wimbledon Championships | |
| Champion |
 Margaret Smith
|
| Runner-up |
 Maria Bueno
|
| Final score | 6–4, 7–5 |
| Draw | 96 (10Q) |
| Seeds | 8 |
Second-seeded Margaret Smith defeated first-seeded Maria Bueno (BRA) 6–4, 7–5 in the final to win the Ladies' Singles tennis title at the 1965 Wimbledon Championships.
The seeded players are listed below. Margaret Smith is the champion; others show the round in which they were eliminated.
Van Zyl, who withdrew because of a family bereavement, was replaced by a Lucky Loser Michelle Boulle (CHI). For the first time in the history of the All England Championships at Wimbledon, there was no British player seeded in the Ladies Singles Championship.
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