Thermococcus celer
| Thermococcus celer | |
|---|---|
| Scientific classification | |
| Domain: | Archaea |
| Phylum: | Euryarchaeota |
| Class: | Thermococci |
| Order: | Thermococcales |
| Family: | Thermococcaceae |
| Genus: | Thermococcus |
| Species: | T. celer |
| Binomial name | |
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Thermococcus celer |
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Thermococcus celer is a Gram-negative, spherical shaped archaeon of the genus Thermococcus. The discovery of Thermococcus celer played an important role in re-rooting the tree of life when it was discovered that T. celer was more closely related to methanogenic Archaea than to other phenotypically similar thermophilic species T. celer was the first archaeon discovered to house a circularized genome. Several type strains of T. celer have been identified: Vu13, ATCC 35543, and DSM 2476.
T. celer was discovered by Dr. Wolfram Zillig in 1983. The organism was isolated on the beaches of Vulcano, Italy from a sulfur rich shallow volcanic crater. Original samples were isolated from the depths of the marine holes and inoculated into 10 mL anaerobic tubes. The tubes contained 100 mg of elemental sulfur as well as a solution of 95% N2 and 5% H2S. The pH was subsequently adjusted to a range of 5-6 through the addition of CaCO3. To ensure that no oxygen had permeated the sample, researchers utilized the oxygen indicator resazurin. Growth was achieved by enrichment with Brock’s Sulfolobus medium, which contains elemental sulfur and yeast, both of which are required by T. celer for optimal growth. Following enrichment, the samples were plated onto polyacrylamide gel and then incubated at 85° Celsius in an anaerobic environment. Once colony growth had been observed, the cells were subjected to centrifugation prior to purification in a TA buffer solution (0.05 mol/1 Tris HCL, 0.022 mol/1 NH4Cl, 0.01 mol/1 β-mercaptoethanol).
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