Second Generation Multiplex Plus

Second Generation Multiplex Plus (SGM Plus), is a DNA profiling system developed by Applied Biosystems. It is an updated version of Second Generation Multiplex. SGM Plus has been used by the UK National DNA Database since 1998.

An SGM Plus profile consists of a list of 10 number pairs, one number pair for each of 10 genetic markers, along with two letters (XX or XY) which show the result of the gender test. Each number pair denotes the two allele values for the marker - one value is inherited from each of the subject's parents. If both alleles are the same, then only a single number, rather than a pair, is recorded.

The genetic markers (or loci) used by SGM Plus are all Short tandem repeats (STRs). The markers used are: VWA, D8S1179, D21S11, D18S51, TH01, FGA, D3S1358, D16S539, D2S1338 and D19S433. Where a marker's designation begins with D, the digits immediately following the D indicate the chromosome that contains the marker. For example, D21S11 is on chromosome 21. SGM Plus also uses the Amelogenin (Amelo) sex-indicating test.

SGM Plus differs from SGM in that SGM does not use the markers D3S1358, D16S539, D2S1338 and D19S433.

SGM Plus has eight markers in common with CODIS FGA, TH01, VWA, D3S1358, D8S1179, D16S539, D18S51, and D21S11. It differs from CODIS in that it uses the additional markers D2S1338 and D19S433 and does not use the five markers CSF1PO, TPOX, D5S818, D7S820, D13S317.

The primers are tagged with the following fluorescent dyes for detection under electrophoresis:

The primers for each locus are arranged on the dyes in the following order, from low molecular weight to large molecular weight:

The dyes to which each primer is attached differ from those of the original SGM DNA profiling system.

The SGM Plus profile of subject GT36865 from a National Institute of Standards and Technology paper is given below:

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