SMIM23

SMIM23 or Small Integral Membrane Protein 23 is a protein which in humans is encoded by the SMIM23 or c5orf50 gene. The longer mRNA isoform is 519 nucleotides which translates to 172 amino acids of a protein. In recent advancements, researchers have identified this gene, along with a few others, could potentially play a role in how facial morphology arises in humans. Though this research is still relatively new it provides the start of a path for further research on this gene.

SMIM23 is a protein-encoding gene. Basic information about its aliases and chromosome location are given in the table. The schematic of the chromosome helps to visualize the location of the gene.

While the gene has two splice isoforms (isoforms X1 and X2), it has three exon/exon boundaries indicating four exons (nucleotide 1-105, 106-157, 158-225, and 226-519).

SMIM23 notably has a transmembrane domain.

The predicted isoelectric point for the unmodified/unprocessed protein in mice is 5.779 while only the transmembrane region in humans has an isoelectric point of 5.928

The gene appears to be Leucine and Glutamic Acid rich though not at any usually high number. It is also weak in all other amino acids besides Alanine, Serine, and Glutamine.

The region underlined in the conceptual translation was predicted to be an Involucrin repeat.

The transmembrane region is 1674.2 Daltons while the whole protein is 200008.51 Da. This is very similar to what was found with UniProt where predicted molecular weight was 20.025 kDa. Antibody kits were investigated to see banding pattern and weight changes that may have occurred post translation. C5orf50 Polyclonal Antibody from ThermoFisher Scientific has a Western Blot banding pattern at 40 kDa. This predicts that there is a significant amount of post-translational modification by addition of large components.

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