The Root River flows for 80 miles (130 km) through the Driftless Area of southeastern Minnesota and is a tributary of the Upper Mississippi River. It is an excellent river for canoeing. The gentle to moderate flowing river drops an average of 3.4 ft/mile from Chatfield, Minnesota, to its pour point in the Mississippi River into Navigation Pool 7 just south of La Crosse, Wisconsin.
The South Branch rises in Mower County as agricultural drainage ditches, which disappear underground, re-emerging as a much cooler stream at Mystery Cave near Preston, Minnesota. The 48°F water creates superb conditions for brook trout.
The river lies within Minnesota's Driftless Area, a region which missed being glaciated during the last ice age, i.e., the Wisconsinian glaciation.
Root River is an English translation of the Dakota-language name.
Many fish species such as brook trout, rainbow trout, brown trout, smallmouth bass, rock bass, channel catfish, crappies, shorthead redhorse, river redhorse, golden redhorse, silver redhorse, greater redhorse, black redhorse, northern hogsuckers, and white suckers inhabit the river. The Root River system provides habitat to a number of bird species. Red-tailed hawks and bald eagles can be found in the area. Blue herons, wild turkeys, and wood ducks are also commonly seen. Numerous mammals can be spotted in the region including deer, gray fox, red fox, coyotes, raccoons, squirrels, and badgers.