Renilla-luciferin 2-monooxygenase
| Renilla-luciferin 2-monooxygenase | |||||||||
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Renilla reniformis luciferase with bound coelenterazine. PDB 2PSD
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| Identifiers | |||||||||
| EC number | 1.13.12.5 | ||||||||
| CAS number | 61869-41-8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / EGO | ||||||||
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| Search | |
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| PMC | articles |
| PubMed | articles |
| NCBI | proteins |
Renilla-luciferin 2-monooxygenase, or RLuc, is a bioluminescent enzyme found in Renilla reniformis, belonging to a group of coelenterazine luciferases. Of this group of enzymes, the luciferase from Renilla reniformis has been the most extensively studied, and due to its bioluminescence requiring only molecular oxygen, has a wide range of applications, with uses as a reporter gene probe in cell culture, in vivo imaging, and various other areas of biological research. Recently, chimeras of RLuc have been developed and demonstrated to be the brightest luminescent proteins to date, and have proved effective in both noninvasive single-cell and whole body imaging.
RLuc catalyzes the chemical reaction
Coelenterazine + O2 coelenteramide + CO2 + hν
In the process, RLuc is oxidized, and a photon of blue light is emitted.
RLuc was isolated from the sea pansy Renilla reniformis, a bioluminescent sea pen. It belongs to the family of oxidoreductases, specifically those acting on single donors with O2 as the oxidant.
The systematic name of this enzyme class is Renilla-luciferin:oxygen 2-oxidoreductase (decarboxylating). Other names in common use include Renilla-type luciferase, aequorin, and luciferase (Renilla luciferin).
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