Luisa Carlotta of Naples and Sicily (Luisa Carlotta Maria Isabella; 24 October 1804 – 29 January 1844), Princess of the Two Sicilies, Infanta of Spain, was a daughter of King Francis I of the Two Sicilies.

Luisa Carlotta was born at the Palace of Portici, the eldest child of King Francis I and of his wife Infanta Maria Isabella of Spain. Her maternal grandfather was King Charles IV of Spain. Luisa Carlotta had eleven younger siblings, including King Ferdinand II of the Two Sicilies.

On 12 June 1819 in Madrid, Luisa Carlotta married her maternal uncle Infante Francisco de Paula of Spain. He was ten years older than Luisa Carlotta. They had eleven children:

Luisa Carlotta wished for her niece Isabella to become heiress to the Spanish throne instead of her uncle Carlos, and therefore supported the new succession law issued by the king. She was involved in an incident during the sickness of the Spanish monarch regarding this issue: when the Spanish king lay sick expecting death (1832), Luisa Carlotta tried to acquire a document forbidding female succession from prime minister Tadeo Calomarde by force and throw it on the fire. When Calomarde tried to save the document, she struck him in the face, upon which he uttered the well known words: "Madame, white hands don't offend!"

When her niece became queen in 1833 with her sister as regent, the rivalry between the sisters worsened and she left Spain for Paris. Luisa Carlotta died at Madrid at the age of 39.

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