Phosphotransferase

Phosphotransferases are a category of enzymes (EC number 2.7) that catalyze phosphorylation reactions. The general form of the reactions they catalyze is:

Where P is a phosphate group and A and B are the donating and accepting molecules, respectively.

Phosphotransferases are generally classified according to the acceptor molecule.

The phosphotransferase system (PTS) is a complex group translocation system present in many bacteria. The PTS transports sugars (such as glucose, mannose, and mannitol) into the cell. The first step of this reaction is phosphorylation of the substrate via phosphotransferase during transport. In the case of glucose, the product of this phosphorylation is glucose-6-phosphate (Glc-6P). Due to the negative charge of the phosphate, this Glc-6P can no longer freely leave the cell. This is the first reaction of glycolysis, which degrades the sugar to pyruvate.

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