PIPES

PIPES
Names

IUPAC name 1,4-Piperazinediethanesulfonic acid (IUPAC)
Other names PIPES
Identifiers
CAS Number	5625-37-6^Y
3D model (Jmol)	Interactive image
ChemSpider	72022^N
ECHA InfoCard	100.024.598
PubChem CID	6992709
InChI InChI=1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)^N Key: IHPYMWDTONKSCO-UHFFFAOYSA-N^N InChI=1/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16) Key: IHPYMWDTONKSCO-UHFFFAOYAG
SMILES C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
Properties
Chemical formula	C₈H₁₈N₂O₆S₂
Molar mass	302.37
Appearance	White powder
Melting point	Decomposes above 300 °C
Boiling point	Decomposes
Solubility in water	1 g/L (100 °C)
Hazards
Main hazards	Irritant
Safety data sheet	External MSDS
NFPA 704	0 1 0
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
N (what is ^YN ?)
Infobox references

PIPES is the common name for piperazine-N,N′-bis(2-ethanesulfonic acid), and frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.

PIPES has pKa (6.76 at 25°C) near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25° C. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues. Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer. It has a negligible capacity to bind divalent ions.

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