Nihonmatsu Domain (二本松藩 Nihonmatsu-han?) was a feudal domain under the Tokugawa shogunate of Edo period Japan, located in southern Mutsu Province. It was centered on Nihonmatsu Castle in what is now the city of Nihonmatsu, Fukushima. For most of its history it was ruled by the Niwa clan. The Nihonmatsu Domain was also the scene of one of the battles of the Boshin War of the Meiji Restoration.
The area around Nihonmatsu was territory of the Hatakeyama clan during the late Kamakura and Muromachi periods. In 1586, Date Masamune destroyed the Hatakeyama and annexed the area to his territories. However, following the Siege of Odawara (1590), Toyotomi Hideyoshi re-assigned the area to Aizu Domain under the rule of the Gamō clan. Hideyoshi later reduced the holdings of the Gamō clan, giving Nihonmatsu and surrounding areas to Asano Nagamasa. This change was very short-lived, as Aizu domain was then reassigned to the Uesugi clan, and their holdings were expanded to encompass Nihonmatsu. The Uesugi were then shifted to Yonezawa Domain following the Battle of Sekigahara by Tokugawa Ieyasu. The Gamō then recovered Nihonmatsu, but the domain was soon beset by a variety of natural disasters, including a massive earthquake, bad weather and flooding, leading to crop failure and widespread famine. This in turn led to a peasant revolt, and the Gamō clan was eventually dispossessed by the Tokugawa shogunate and sent to Iyo Province in Shikoku.