Nicholas Wotton (c. 1497 – 26 January 1567) was an English diplomat, cleric and courtier.
He was a son of Sir Robert Wotton of Boughton Malherbe, Kent, and a descendant of Sir Nicholas Wotton, Lord Mayor of London in 1415 and 1430, who was Member of Parliament for the City from 1406 to 1429.
Soon after ordination Wotton was granted the benefices of Boughton Malherbe and of Sutton Valence, and later of Ivychurch, Kent. Desirous of a more worldly career, he entered the service of Prince-Bishop Cuthbert Tunstall, then Bishop of London. Having helped to draw up the Institution of a Christian Man, Wotton in 1539 went to arrange the marriage between Henry VIII and Anne of Cleves and the union of Protestant princes which was to be the complement of this union. Wotton crossed over to England with the new royal bride but, unlike Thomas Cromwell, he did not lose the royal favour when the king repudiated Anne.
In 1541, having already refused the bishopric of Hereford, he became the first post-Reformation dean of Canterbury and in 1544 dean of York. In 1543 he went on diplomatic business to the Netherlands, and for the next year or two he had much intercourse with the Emperor Charles V He helped to conclude the Treaty of Ardres between England and France in 1546, and was Ambassador resident in France from 1546 to 1549. Henry VIII made Wotton an executor of his Will and left him £300, and in October 1549, under Edward VI the post of Minister of State lay vacant; he held the post for about a year until succeeded by the unimpeachable Protestant Sir William Cecil.