The Mytilenian Debate (also spelled "Mytilenean Debate" or "Mytilenaean Debate") in the Athenian Assembly concerned reprisals against the city-state of Mytilene, which had attempted unsuccessfully to shake off Athenian hegemony, during the Peloponnesian War. The Debate occurred in 427 B.C.;Thucydides reports it in book three of his History of the Peloponnesian War, and uses the events and the speeches as a major opportunity to reflect and to offer his views on the political and ideological impact of the war on the parties involved.
Mytilene was one of the last non-tributary members of the Delian League which chose to man their own warships and send subjects to fight alongside the Athenian fleet. Mytilene feared tributary democracy and did not want to be reduced to the status of other Athenian allies. In addition, Mytilene was different from most allies because it was ruled by an oligarchy. Mytilene had contemplated ending its alliance with Athens, but Sparta had rejected her appeal for an alliance to enable this. However, this rejection had been prior to the outbreak of the Peloponnesian War, which created an opportunity for the Mytilenians, who sent an envoy to Olympia in 428 B.C. to seek assistance from the Peloponnesians. The Mytilenians asked for direct help in Mytilene, but also for an invasion of Attica, pointing out that since Athens had recently been devastated by a plague it would have financial difficulties fighting on two fronts.
Thucydides represents the Mytilenian ambassadors at Olympia as arguing that Athens had taken advantage of the Delian League and used it as a mechanism for "enslaving" and exploiting other Greeks; that it was only a matter of time before they set their sights on Mytilene’s prosperous and autonomous island of Lesbos and forced it into their system of oppression; and that the Mytilenians could not wait for this and should pre-emptively break away before Athens forced her will upon them. The Peloponnesian League agreed to aid Mytilene.
It has been pointed out that these arguments are silent on a major motivation of the oligarchs towards rebellion. The Mytilenian government sought the unification of the five city-states of Lesbos under their leadership.; Athenian policy aimed to break up larger units within the confederacy and would not support any enhancements in Mytilenian power.