Maria Isabelle of Naples and Sicily (2 December 1793 – 23 April 1801) was a member of the French Royal Family (branched out to the Kingdom of the Two Sicilies). She was styled Princess of Naples and Sicily.

Maria Isabelle was born in Naples, and she was named after her paternal aunt Maria Isabel Ana, who died at the age of six in 1749. Her father was Ferdinand, Duke of Calabria, the third son and ninth child of King Charles III of Spain and Queen Maria Amalia of Saxony. Her mother was Maria Carolina, Archduchess of Austria, the tenth daughter and thirteenth child of the famous Empress Maria Theresa of Austria and her husband, Francis I, Holy Roman Emperor. Through her mother she was a niece of Marie Antoinette; through her father she was a niece of Maria Luisa of Spain and Charles IV of Spain. She was then styled Princess of Naples and Sicily.

Her brothers included the future King Francis and Leopold, Prince of Salerno. Another, Carlo, Duke of Calabria died in 1778 aged 3 of smallpox. Her older sisters included Princess Maria Theresa, namesake of her grandmother and Princess Luisa, future Grand Duchess of Tuscany. Her younger sister Princess Maria Cristina, was the wife of the future Charles Felix of Sardinia as Queen of Sardinia. Maria Cristina's twin Princess Maria Cristina Amelia died in 1783 of smallpox. Another sister was the Queen of the French as the wife of Louis Philippe I and the youngest was the future Princess of Asturias.

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