The MS-DOS API is an API which originated with 86-DOS and is used in MS-DOS/PC DOS and other DOS-compatible operating systems. Most calls to the DOS API are invoked using software interrupt 21h (INT 21h). By calling INT 21h with a subfunction number in the AH processor register and other parameters in other registers, one invokes various DOS services. DOS services include keyboard input, video output, disk file access, executing programs, memory allocation, and various other things. In the late 1980s, DOS extenders along with the DOS Protected Mode Interface (DPMI) allow the programs to run in either 16-bit or 32-bit protected mode and still have access to the DOS API.
The original DOS API in 86-DOS and MS-DOS 1.0 was designed to be functionally compatible with CP/M. Files were accessed using file control blocks (FCBs). The DOS API was greatly extended in MS-DOS 2.0 with several Unix concepts, including file access using file handles, hierarchical directories and device I/O control. In DOS 3.1, network redirector support was added. In MS-DOS 3.31, the INT 25h/26h functions were enhanced to support hard disks greater than 32 MB. MS-DOS 5 added support for using upper memory blocks (UMBs). After MS-DOS 5, the DOS API was unchanged for the successive standalone releases of DOS.
In Windows 9x, DOS was generally used as a bootloader which loaded the protected-mode operating system and graphical shell. DOS was usually accessed from a virtual DOS machine (VDM) but it was also possible to boot directly to real mode MS-DOS 7.0 without loading Windows. The DOS API was extended with enhanced internationalization support and long filename support, though the long filename support was only available in a VDM. With Windows 95 OSR2, DOS was updated to 7.1, which added FAT32 support, and functions were added to the DOS API to support this. Windows 98 and Windows ME also implement the MS-DOS 7.1 API, though Windows ME reports itself as MS-DOS 8.0.