Loyalty and Betrayal is the sixth studio album by American rapper E-40, released October 10, 2000 on Jive and Sick Wid It Records. The album features production by Battlecat, Bosko, Jazze Pha, Rick Rock and Tone Capone. It peaked at number 4 on the Billboard Top R&B/Hip-Hop Albums and at number 18 on the Billboard 200. Two singles were released, "Behind Gates" and "Nah, Nah...", the latter peaked at number 61 on the Billboard Hot R&B/Hip-Hop Songs. The album features guest performances by fellow members of The Click: B-Legit, D-Shot and Suga-T, as well as Mack 10, Eightball, Too Short, Young Mugzi, Levitti, Pimp C and Birdman.

Along with a single, a music video was produced for the song "Behind Gates", featuring Ice Cube and a cameo appearance by WC. A second single, "Nah, Nah...", was also released as a music video, featuring Nate Dogg and cameo appearances by Ant Banks, JT the Bigga Figga, Mac Shawn and The Click.