Throughout its history, the Portuguese monarchy has had only two queens regnant: Maria I and Maria II of Portugal (and, arguably, Beatriz for a short period of time in the 14th century).
The other women who used the title of "Queen of Portugal" were merely queens consort, wives of the Portuguese kings. Nevertheless, many of them were highly influential in the country's history, having ruled as regents for their minor children and heirs, as well has having a great influence over their spouses.
Elizabeth of Aragon, who was married to Dinis I, was made a saint after there were said to have been miracles performed after her death.
The husband of a Portuguese Queen Regnant could only be titled King after the birth of any child from that marriage. Portugal had two Princes Consort - Auguste de Beauharnais, 2nd Duke of Leuchtenberg and Ferdinand of Saxe-Coburg and Gotha - both consorts to Maria II of Portugal. The first one died leaving his wife childless, and therefore never became King of Portugal. Maria II's second husband was her consort until the birth of their first child, Pedro V of Portugal. Upon birth of the heir, Ferdinand ceased to be consort and instead became de jure uxoris King of Portugal, as Fernando II. Maria I's husband, Pedro III, was titled de jure uxoris King automatically after his wife's accession, since the couple already had an heir José, Prince of Beira.