This is a partial list of characters in Jungle Jam and Friends: The Radio Show!.
The narrator (Nathan Carlson), often referred to as "Mr. Narrator" by the characters, is an interactive force in the jungle; there is some indication that he is a jungle explorer.
Gruffy Bear (Nathan Carlson), the de facto main character of the series, is a jovial, level-minded construction worker brown bear. He is the only primary character with a wife (Muffy), a child (Gruffy, Jr), and a dog (Wuffy).
Sully the Aardvark (Nathan Carlson) is a simple-minded aardvark. Frequently noted as an excellent cannon ball diver, he is the best friend of Millard J. Monkey. At one point he played the triangle in the Jungle Band.
Millard Jackanapes Monkey (Phil Lollar), usually referred to as Millard J. Monkey or Millard the Monkey, is a clumsy, foolhardy monkey and the best friend of Sully the Aardvark. Millard was a later edition to the cast of characters, but quickly became a central character and fan favorite.
Nozzles the Elephant (Nathan Carlson) is a usually wise, brooding African elephant. However, he is prone to giving in to the other animals' inanity. A lover of mystery novels and gadgets.
Racquet the Skunk (Nathan Carlson) is a kind-hearted but ambitious skunk. He is frequently shown to be an excellent badminton player.
Jean-Claude the Flying Squirrel (Phil Lollar) is a somewhat self-centered French flying squirrel. Ironically, he pilots a biplane.
Cheetah Bonita, Cheetah Anita, and Cheetah Sally (Julie Miller) are members of the musical group The Cheetah Sisters.
The sloth (Cindy Parker), also referred to as Sloth, is an easy-going, female sloth.