Johnstone Strait is a 110 km (68 mi) channel along the north east coast of Vancouver Island in British Columbia, Canada. Opposite the Vancouver Island coast, running north to south, are Hanson Island, West Cracroft Island, the mainland British Columbia Coast, Hardwicke Island, West Thurlow Island and East Thurlow Island. At that point, the strait meets Discovery Passage which connects to Georgia Strait.
The strait is between 2.5 km (1.6 mi) and 5 km (3.1 mi) wide. It is a major navigation channel on the west coast of North America. It is the preferred channel for vessels from the Georgia Strait leaving to the north of Vancouver Island through the Queen Charlotte Strait bound for Prince Rupert, Queen Charlotte Islands, Alaska, and the North Pacific Ocean, and for southbound vessels from those areas bound for the Port of Vancouver.
The Strait is home to approximately 150 orca whales during the summer months, which are often seen by kayakers and boaters packed with tourists. Scientists including Michael Bigg and Paul Spong have been researching the orcas in the Strait since 1970. Spong established the OrcaLab, based on studying the Orcas in their natural habitat without interfering with their lives or their habitat. The strait includes the Robson Bight (Michael Bigg) Ecological Reserve.