Johan Ludvig Holstein, Lensgreve of Ledreborg (7 September 1694 – 29 January 1763) was a Danish Minister of state from 1735 to 1751. The Danish colony Holsteinsborg on Greenland (now Sisimiut), was named after him.
He was the ancestor of the Holstein-Ledreborg family, including Ludvig Holstein-Ledreborg and Knud Johan Ludvig, Lensgreve Holstein til Ledreborg, husband of Princess Marie Gabriele of Luxembourg.
In 1739 he built Ledreborg Manor near Lejre, Denmark.
Johan Ludvig was the son of Johan Georg Holstein, who would himself become Danish prime minister, and Ida Frederikke Joachime of the Bülow family. The birth took place at the Lübz castle which belonged to his maternal grandmother. His tutors during his upbringing included J. W. Schröder who later would go on to tutor Christian VI of Denmark. In 1711 his father sent him to Hamburg where he studied with Johann Albert Fabricius for a year. Subsequently, he studied and traveled at various places in Germany, the Netherlands, France and England until 1716, when he became a courtier and civil servant in Denmark.
He obtained the position of marshal of the court to Crown Prince Christian (later King Christian VI) in 1721. In 1727, he became a member of the College of Missions where his father was president, and the same year he also became director of the Waisenhuset, an orphanage King Frederick IV founded that year.
When Christian VI became king in 1730, he appointed Holstein stiftsamtmand (amtmann and co-administrator of the diocese with the bishop) of Zealand and amtmann of Copenhagen and Roskilde. In February 1734, he was appointed Minister of Finance. As the new king soon became disappointed in the men he originally had chosen to lead the chanceries, he appointed Holstein leading secretary of the Danish chancery (a position often known as Minister of State) on 12 May 1935, replacing Iver Rosenkrantz. At the same time, he became member of the king's council (Konseillet). In 1740, he also replaced Rosenkrantz as patron of the University of Copenhagen. In 1757, he additionally became first member of a special agricultural committee which was operative for several years.