James Melville (26 July 1556 – 1614) was a Scottish divine and reformer, son of the laird of Baldovie, in Forfarshire. He was educated at Montrose and St Leonard's College, St Andrews.
In 1574 he proceeded to the University of Glasgow. There his uncle, Andrew Melville, the reformer and scholar, was principal. Within a year James became one of the regents.
When, in 1580, Andrew became Principal of St Mary's College, St Andrews (then called New College), James accompanied him, and acted as Professor of Hebrew and Oriental Languages. For three and a half years he lectured in the university, chiefly on Hebrew, but he had to flee to Berwick in May 1584 (a few months after his uncle's exile) to escape the attacks of his ecclesiastical enemy, Bishop Patrick Adamson. After a short stay there and at Newcastle-on-Tyne, and again at Berwick, he proceeded to London, where he joined some of the leaders of the Scottish Presbyterian party.
The taking of Stirling Castle in 1585 having changed the political and ecclesiastical positions in the north, he returned to Scotland in November of that year, and was restored to his office at St Andrews. From 1586 to his death he took an active part in Church controversy.
In 1589 he was moderator of the General Assembly and on several occasions represented his party in conferences with the court. Despite his antagonism to James's episcopal schemes, he appears to have won the king's respect. He answered, with his uncle, a royal summons to London in 1606 for the discussion of Church policy.
The uncompromising attitude of the kinsmen, though it was made the excuse for sending the elder to the Tower, brought no further punishment to James than easy detention within ten miles of Newcastle-on-Tyne. During his residence there it was made clear to him by the king's agents that he would receive high reward if he supported the royal plans. In 1613 negotiations were begun for his return to Scotland, but his health was broken, and he died at Berwick in January 1614.