Inhibitor of caspase-activated DNase

DFFA
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1IBX, 1IYR, 1KOY
Identifiers
Aliases	DFFA, DFF-45, DFF1, ICAD, DNA fragmentation factor subunit alpha
External IDs	MGI: 1196227 HomoloGene: 3240 GeneCards: DFFA
Gene location (Human) Chr. Chromosome 1 (human) Band 1p36.22 Start 10,456,522 bp End 10,472,526 bp
Gene location (Mouse) Chr. Chromosome 4 (mouse) Band 4|4 E2 Start 149,104,146 bp End 149,120,647 bp
RNA expression pattern More reference expression data
Gene ontology Molecular function • protein binding • protein domain specific binding • protein binding involved in protein folding • deoxyribonuclease inhibitor activity Cellular component • nuclear chromatin • cytosol • lipid droplet • intracellular • cell nucleus • nucleoplasm • cytoplasm • plasma membrane Biological process • positive regulation of apoptotic process • negative regulation of execution phase of apoptosis • negative regulation of apoptotic DNA fragmentation • apoptotic DNA fragmentation • thymocyte apoptotic process • apoptotic process • negative regulation of deoxyribonuclease activity • chaperone-mediated protein folding Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	1676	13347
Ensembl	ENSG00000160049	ENSMUSG00000028974
UniProt	O00273	O54786
RefSeq (mRNA)	NM_213566 NM_004401	NM_001025296 NM_010044
RefSeq (protein)	NP_004392 NP_998731 NP_998731.1	NP_001020467 NP_034174
Location (UCSC)	Chr 1: 10.46 – 10.47 Mb	Chr 4: 149.1 – 149.12 Mb
PubMed search

DNA Fragmentation factor 45kDa, C terminal domain
nmr structure of dff-c domain
Identifiers
Symbol	DFF-C
Pfam	PF09033
InterPro	IPR015121
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

1IBX, 1IYR, 1KOY

1676

13347

ENSG00000160049

ENSMUSG00000028974

O00273

O54786

NM_213566
NM_004401

NM_001025296
NM_010044

NP_004392
NP_998731
NP_998731.1

NP_001020467
NP_034174

DNA fragmentation factor subunit alpha (DFFA), also known as Inhibitor of caspase-activated DNase (ICAD), is a protein that in humans is encoded by the DFFA gene.

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.