Horio Yoshiharu (堀尾 吉晴 1542 – July 26, 1611?) was a Japanese daimyo during the Azuchi-Momoyama and Edo periods. He was appointed to the position of one of three chūrō (arbiters) by Toyotomi Hideyoshi and was the first leader of the Matsue clan. He was also known as Horio Mosuke (堀尾 茂助).
After wrestling a wild boar at a young age, Toyotomi Hideyoshi learned of it and brought Yoshiharu into his service.
Yoshiharu served Hashiba Hideyoshi (Toyotomi Hideyoshi) when Oda Nobunaga attacked Inabayama Castle. When Hideyoshi attacked Bitchu Takamatsu Castle, Yoshiharu conducted an inquest into the death of Shimizu Muneharu. Horio was involved in various battles such as the Battle of Yamazaki and shined as a retainer of Hideyoshi. At the Battle of Shizugatake in 1583, he killed Saito Toshikazu after he attempt to scroll past Hideyoshi's lines. In 1590, Hideyoshi awarded him 120,000 koku at Hamamatsu, Tōtōmi Province because of the credit for the siege of Odawara. Horio Yoshiharu, Nakamura Kazuuji and Ikoma Chikamasa were appointed the three chūrō by Hideyoshi in his last years, and participated in the Toyotomi administration.