Hemichrome
A hemichrome (FeIII) is a form of low-spin methemoglobin (metHb). Hemichromes, which precede the denaturation processes of haemoglobin (Hb), are mainly produced by partially denaturated haemoglobins and form histidine complexes. Hemichromes are usually associated with blood disorders.
Hemichromes can be classified in two main categories: reversible and irreversible.
Reversible hemichromes (Hch-1) have the ability to return to their native formation (haemoglobin). Some hemichromes can be reduced to the high-spin state of deoxyhaemoglobin, while others are first being reduced to hemochromes (FeII) and then to deoxyhaemoglobin through anaerobic dialysis. Photolysis, in the presence of oxygen from CO and its reaction with the hemochrome, can quickly convert a hemichrome to oxyhaemoglobin (HbO2).Irreversible hemichromes (Hch-2) cannot be converted to their native form.
Both the reversible and irreversible hemichromes have a similar rate during proteolytic degradation and they both have a lower percentage of alpha helixes.
Upon blood exiting the body, haemoglobin in blood transits from bright red to dark brown, which is attributed to oxidation of oxy-hemoglobin (HbO2) to methemoglobin (met-Hb) and ending up in hemichrome (HC). For forensic purposes, the fractions of HbO2, met-Hb and HC in a bloodstain can used for age determination of bloodstains when measured with Reflectance Spectroscopy [1].
Hemichromes form an insoluble macromolecule (macromolecular aggregate) by copolymerization with the cytoplasm of band 3. Covalent bonds reinforce the aggregate interactions of the hemichromes which are accumulated on the surface of the membrane. However, hemichromes are less stable than their native form.
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