Halostagnicola larsenii
| Halostagnicola larsenii | |
|---|---|
| Scientific classification | |
| Domain: | Archaea |
| Kingdom: | Euryarchaeota |
| Phylum: | Euryarchaeota |
| Class: | Halobacteria |
| Order: | Halobacteriales |
| Family: | Halobacteriaceae |
| Genus: | Halostagnicola |
| Binomial name | |
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Halostagnicola larsenii Strain XH-48 Castillo et al. 2006 |
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| Species | |
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Halostagnicola larsenii is a non-motile, aerobic, gram-negative, rod shaped archaeon. It is a halophilic, neutrophilic, chemo-organotroph and was isolated from samples taken from a saline lake in China. The etymology of the name comes from Halos Greek for salt, stagnum Latin for standing water, cola Latin for inhabitant or dweller, and Larsenii named after the Norwegian microbiologist, Helge Larsen, who was a pioneer in research regarding halophiles.
In September 2003, researchers from the University of Seville, Spain, obtained samples of sediment from a lake in Inner Mongolia, China. Lake Xilinholt is an extremely saline lake, thus providing the optimum growth conditions for Halostagnicola larsenii . The samples were cultivated in a 20% saline solution.Nutrient agar plates were used to cultivate the samples. The media contained sodium chloride and was optimized at a pH of 7.5.H. larsenii grows optimally at 15% NaCl, 37 ° Celsius and pH 7-8. It is unable to grow at temperatures above 50 ° Celsius. Further characterization of the species was conducted and it was proposed by Castillo et al., that strain XH-48 be identified as a new species within the Halostagnicola phylum.
Morphology was determined using phase contrast optics by an Olympus BX41 microscope. Cells of Halostagnicola larsenii XH48 are 0.5-1.0 micrometers wide and 1.0-3.0 micrometers long. Cells of H. larsenii are pleomorphic, and display rod, square or disc shaped cells. This reflects the strain's ability to change size and shape in response to changes in the environment, such as salinity. The colony morphology of H. larsenii is circular, smooth, opaque and pink in color. Polar ether lipids found in its membrane include phosphatidylglycerol and phosphatidylglyceromethylphosphate. These lipids were extracted with chloroform and methanol. Tests revealed this organism is oxidase positive and catalase negative.
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