Guido Ubaldo Abbatini (1600–1656) was an Italian painter of the Baroque period, active mainly in Rome and Usigni.
Guido Ubaldo Abbatini was a pupil of the painter Giuseppe Cesari, and later worked under Pietro da Cortona. He frequently painted in fresco. He was born in Città di Castello and died in Rome. He painted the ceiling of the chapel of Santa Theresa in Santa Maria della Vittoria in Rome.