Girolamo Colonna (23 March 1604 – 4 September 1666) was an Italian Cardinal of the Roman Catholic Church and member of the noble Colonna family.
Colonna was born at Orsogna into the Colonna family and his extended family included members of various 17th-century Italian royal and noble houses. His father was Filippo I Colonna, Prince of Paliano and his mother was Lucrezia Tomacelli. His sister, Anna Colonna, married Taddeo Barberini, brother of cardinals Francesco Barberini and Antonio Barberini (Antonio the Younger).
Colonna was uncle to the children of his sister and brother-in-law, Maffeo Barberini, Cardinal Carlo Barberini and Lucrezia Barberini who married Francesco I d'Este, Duke of Modena.
He was elevated to cardinal on 30 August 1627 by Barberini Pope Urban VIII (the uncle of his brother-in-law Taddeo) at the insistence of his sister, Anna.
After the death of Pope Urban, he participated in the Papal conclave of 1644 that elected Pope Innocent X. He was Archpriest of the patriarchal Lateran basilica and Legate a latere for the opening and closing of the Holy Door in the patriarchal Lateran basilica in the Holy Year of 1650. He took part in the conclave of 1655 that elected Pope Alexander VII.