Gertrude Anne of Hohenberg (c. 1225 – 16 February 1281) was German queen from 1273 until her death, by her marriage with King Rudolf I of Germany. As queen consort, she became progenitor of the Austrian House of Habsburg.

Gertrude was born in Deilingen, Swabia to Count Burkhard V of Hohenberg (died 1253) and his wife Matilda (Mechtild), daughter of Count Palatine Rudolf II of Tübingen. The comital Hohenberg dynasty, a cadet branch of the Swabian House of Hohenzollern, then ruled over extended estates in southwestern Germany. Citing contemporary sources, Gertrude's descent was questioned by the Swiss historian Aegidius Tschudi (1505–1572), who postulated a Frohburg lineage; nevertheless, his objections have been disproved.

About 1251 in Alsace, Gertrude married Rudolf (1218–1291), son of Count Albert IV of Habsburg and Heilwig of Kyburg. She went on to live with her husband as a comital couple in Rheinfelden. They had eleven children:

Gertrude's husband was elected King of the Romans (as Rudolf I) in Frankfurt on 29 September 1273. The election was largely due to the efforts of her cousin Burgrave Frederick III of Nuremberg. Rudolf was crowned in Aachen Cathedral on 24 October 1273. As "Queen Anne" (Anna Regina) she served as his consort for the following eight years. Reluctant to interfere in politics, she witnessed Rudolf's struggles to secure his rule against the rivalling King Ottokar II of Bohemia, as well as his fruitless efforts to be crowned Holy Roman Emperor.

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