Lucius Junius Gallio Annaeanus or Gallio was a Roman senator and brother of famous writer Seneca. He is best known for his impartial judgment of a legal case involving Paul the Apostle in Corinth.
Gallio (originally named Lucius Annaeus Novatus) was the son of the rhetorician Seneca the Elder and the elder brother of Seneca the Younger, was born in Corduba (Cordova) c. 5 BC. He was adopted by Lucius Junius Gallio, a rhetorician of some repute, from whom he took the name of Junius Gallio. His brother Seneca, who dedicated to him the treatises De Ira and De Vita Beata, speaks of the charm of his disposition, also alluded to by the poet Statius (Silvae, ii.7, 32). It is probable that he was banished to Corsica with his brother, and that they returned together to Rome when Agrippina selected Seneca to be tutor to Nero. Towards the close of the reign of Claudius, Gallio was proconsul of the newly constituted senatorial province of Achaea, but seems to have been compelled by ill-health to resign the post within a few years. He was referred to by Claudius as "my friend and proconsul" in the Delphi Inscription circa 52.
Gallio was a suffect consul in the mid-50s and Cassius Dio records that he introduced Nero's performances. Not long after the death of his brother, Seneca, Gallio (according to Tacitus, Ann. 15.73) was attacked in the Senate by Salienus Clemens, who accused him of being a "parricide and public enemy", though the Senate unanimously appealed to Salienus not to profit "from public misfortunes to satisfy a private animosity". He did not survive this reprieve long. When his second brother, Annaeus Mela, opened his veins after being accused of involvement in a conspiracy (Tacitus, Ann. 16.17), Gallio seems to have committed suicide, perhaps under instruction in 65 at the age 64.