Francis Holcroft (1629?–1693) was an English ejected minister.
He was son of Sir Henry Holcroft, born at West Ham in Essex. He matriculated at Clare Hall, Cambridge in 1647.John Tillotson was his chamber-fellow about 1650. While at Cambridge he embraced puritan principles, and became a communicant with the congregation of Jonathan Jephcot at Swaffham Priors. He graduated M.A. in 1654, was elected fellow of his college, and took holy orders.
For some years he voluntarily supplied the parish of Litlington, Cambridgeshire. About 1655 he accepted the living of Bassingbourne, Cambridgeshire, where he was a successful preacher, and, was assisted by the Rev. Joseph Oddy, fellow of Trinity College, Cambridge. Holcroft eventually formed a church on congregational principles, and, after being ejected in 1662 from Bassingbourne, became a bitter opponent of episcopalianism. After his ejectment, he brought his former parishioners into congregations at convenient centres, and acted as their minister, with the assistance of Oddy and S. Corbyn, both ejected fellows of Trinity College, Cambridge, who were appointed at a general meeting at Eversden.
In 1663 Holcroft was imprisoned in Cambridge gaol, by order of Sir Thomas Chickley, for illegal preaching, but he was occasionally allowed by the warder to visit his congregations. At the assizes he was sentenced to abjure the realm, but on Arthur Annesley, 1st Earl of Anglesey representing his case to Charles II he was allowed to remain in gaol. He was released at the Declaration of Indulgence in 1672, returned to his preaching, and was again imprisoned. By means of a writ of certiorari he was removed as an insolvent debtor to the Fleet Prison in London, and frequently preached there to large crowds of people. On discharging his debts he was released.