Florence Jean Castleberry , better known to all as "Flo", is a fictional character in the movie Alice Doesn't Live Here Anymore, the subsequent television series, Alice, and that show's spinoff, Flo. In the movie, Flo was played by actress Diane Ladd (who would later play Flo's replacement, Isabelle Amanda Dupree on the TV series), and on the television series she was played by Polly Holliday.
Florence Jean Castleberry was born in the small community of Cowtown, Texas, to Edsel Jarvis (Forrest Tucker) and Velma Castleberry (Sudie Bond). In many ways Velma had been a raucous woman herself; and that was how Flo became who she was. She also had three younger brothers - Jimmie Joe (Burton Gilliam), Lonnie (G.W. Bailey), Rhett (Robert Ayers), and a younger sister named Fran (Lucy Lee Flippin). Jimmie Joe fell madly in love with Alice in the episode of "Alice", "Love Me, Love My Horse". Lonnie and Rhett appeared in an episode of "Flo", "A Castleberry Thanksgiving". Fran and Flo were opposites in every way. Where Flo was outgoing and graceful, Fran was introverted and clumsy. Fran, who worked at the Department of Motor Vehicles, was forever engaged to a feed store worker named Wendell Tubbs. Whilst in Cowtown, Flo had worked as a waitress, and was considered one of the best.
Her best friend in Cowtown was a woman named Miriam Willoughby (Joyce Bulifant).
Flo dropped out of school at age 16 to get married. After three extremely unsuccessful marriages, (all three were in front of Justices of the Peace, she said that those three quickies didn't seem to take). In the film Alice Doesn't Live Here Anymore, Flo was depicted as being married -- albeit a rather unhappy marriage -- and the mother of a preteen daughter; these elements were retrospectively amended in the television version, with Flo now portrayed as thrice divorced, and never having had children. Flo became a waitress at Mel's Diner in Phoenix, Arizona, a roadside diner run by one Mel Sharples.