MTA Regional Bus Operations operates local and express buses serving New York City in the United States out of 29 bus depots. These depots are located in all five boroughs of the city, with one located in nearby Yonkers in Westchester County. 21 of these depots serve MTA New York City Transit (NYCT)'s bus operations, while the remaining eight serve the MTA Bus Company (the successor to private bus operations taken over in the 2000s). Theses facilities perform regular maintenance, cleaning, and painting of buses, as well as collection of revenue from bus fareboxes. Several of these depots were once car barns for streetcars, while others were built much later and have only served buses. Employees of the depots are represented by local divisions of the Transport Workers Union of America (TWU), particularly the TWU Local 100, or of the Amalgamated Transit Union (ATU).
On June 1, 1940, the New York City Board of Transportation (BOT) took over the streetcar operations of the Brooklyn–Manhattan Transit Corporation (BMT), as part of the unification of the city's transit system under municipal operations. The streetcar lines would be motorized into diesel bus routes or trolleybus routes over the next two decades. In 1947, the BOT took over the North Shore Bus Company in Queens and Isle Transportation in Staten Island, giving the city control of the majority of surface transit in Brooklyn, Queens, and Staten Island. On September 24, 1948, the BOT took over the East Side Omnibus Corporation and Comprehensive Omnibus Corporation in Manhattan, receiving two depots in East Harlem. From 1947 to 1950, the BOT reconstructed numerous depots and trolley barns inherited from the private operators, and erected or purchased new facilities to expand capacity. In 1962, the New York City Transit Authority (successor to the BOT) and its subsidiary Manhattan and Bronx Surface Transit Operating Authority (MaBSTOA) took over the operations of the Fifth Avenue Coach Company in Manhattan and the Bronx. The Transit Authority inherited at least 12 bus depots from the company, some of which were kept in operation while others were condemned and closed. From 2005 to 2006, the remaining private operators were taken over by the MTA Bus Company. The MTA inherited eight facilities at this time, which had been built either by the companies or the New York City Department of Transportation (NYCDOT).