The Evansville, Owensboro and Nashville Railroad was a 19th-century railway company in western Kentucky in the United States. It operated from 1873, when it purchased the Owensboro & Russellville, until 1877, when it was purchased by the Owensboro & Nashville. Its former rights-of-way currently form parts of the class-I CSX Transportation railway.
It connected with the Elizabethtown and Paducah Railroad and its successors – the Louisville, Paducah and Southwestern Railroad and the Paducah and Elizabethtown Railroad – at Central City in Muhlenberg County.