Sir Edward Lewknor or Lewkenor (1542 – 19 September 1605) was an important Puritan voice in the English Parliament through the later reign of Queen Elizabeth I.
Edward was the eldest son of the courtier Edward Lewknor of Kingston Buci, Sussex, who died after three months in the Tower of London in September 1556 under deferral of execution, attended by his wife Dorothy (sister of Sir Thomas Wroth) - Sir Edward's mother - and one of his daughters. He had been formally convicted of using his situation at Court to procure a copy of King Henry VIII's will, to assist the conspiracy of Henry Dudley and Henry Peckham against Queen Mary I.
Edward was aged 14 when his father was buried within the Tower precinct. Many lands, including the manor and advowson of Hamsey, East Sussex, were restored to his mother in February 1556/57 by Mary's Letters Patent. In the first year of Elizabeth an act was passed, on the petition of Lewknor's four sons (Edward, Thomas, Stephen and William) and six daughters (Jane, Maria, Elizabeth, Anne, Dorothie and Lucrece) to restore them to their blood, lineage and degree. This restored all their ancestral hereditaments excepting those held in use, possession or reversion by their father at the time of his treason and attainder, or any which either Mary or Elizabeth should have found cause to withhold. They were therefore entitled to make their pedigrees as Lewknor's heirs as if he had never been attainted, and to make conveyances thereof, except of lordships, honours and other benefits to which their Majesties were entitled on account of the attainder.