EcoRII
| Restriction endonuclease EcoRII, N-terminal | |||||||||
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crystal structure of restriction endonuclease ecorii mutant r88a
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| Identifiers | |||||||||
| Symbol | EcoRII-N | ||||||||
| Pfam | PF09217 | ||||||||
| Pfam clan | CL0405 | ||||||||
| InterPro | IPR015300 | ||||||||
| SCOP | 1na6 | ||||||||
| SUPERFAMILY | 1na6 | ||||||||
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| Available protein structures: | |
|---|---|
| Pfam | structures |
| PDB | RCSB PDB; PDBe; PDBj |
| PDBsum | structure summary |
| EcoRII C terminal | |||||||||
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crystal structure of restriction endonuclease ecorii mutant r88a
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| Identifiers | |||||||||
| Symbol | EcoRII-C | ||||||||
| Pfam | PF09019 | ||||||||
| Pfam clan | CL0236 | ||||||||
| InterPro | IPR015109 | ||||||||
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| Available protein structures: | |
|---|---|
| Pfam | structures |
| PDB | RCSB PDB; PDBe; PDBj |
| PDBsum | structure summary |
Restriction endonuclease (REase) EcoRII (pronounced "eco R two") is an enzyme of restriction modification system (RM) naturally found in Escherichia coli, a Gram-negative bacteria. Its molecular mass is 45.2 kDa, being composed of 402 amino acids.
EcoRII is a bacterial Type IIEREase that interacts with two or three copies of the pseudopalindromic DNA recognition sequence 5'-CCWGG-3' (W = A or T), one being the actual target of cleavage, the other(s) serving as the allosteric activator(s). EcoRII cut target DNA sequence CCWGG generating sticky ends.
The apo crystal structure of EcoRII mutant R88A (PDB: 1NA6) has been solved at 2.1 Å resolution. The EcoRII monomer has two domains, N-terminal and C-terminal, linked through a hinge loop.
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