The Amboise conspiracy, also called Tumult of Amboise, was a failed attempt by Huguenots in 1560 to gain power over France by abducting the young king Francis II and arresting Francis, Duke of Guise and his brother, the Cardinal of Lorraine. It was one of the events directly leading up to the Wars of Religion that divided France from 1562 to 1598.
At the death of Henry II in 1559, the Protestants of France looked forward to a relaxation of stringent policies against their religion, but the young king, Francis II, retained his father's policy. Among the king's advisors were his wife's uncles, Francis, Duke of Guise and Charles of Guise, Cardinal of Lorraine, who through their niece exercised great influence with the King.
A group of provincial aristocrats decided to take matters into their own hands, by kidnapping the King and arresting the brothers Guise. Chief among the conspirators was Godefroy de Barry, seigneur de La Renaudie, of Périgord.
La Renaudie gathered round him like-minded Huguenot gentlemen representing various regions of France: Charles de Castelnau de Chalosse, Bouchard d'Aubeterre, Edme de Ferrière-Maligny (brother of Jean de Ferrieres), Captains Mazères, Cañizares, Sainte-Marie and Lignières, Jean d'Aubigné (father of Agrippa d'Aubigné) and Ardoin de Porcelet. Paulon de Mauvans, whose brother had been executed, rallied the Huguenots of Provence at Mérindol, 12 February 1560, promised 2,000 men and sent 100 to Nantes.Gaspard de Coligny, later also a leading Huguenot, discouraged the nobles of Normandy from involving themselves in the plot. Leading Protestant bourgeois of Orléans, Tours and Lyon were apprised of developments.